Extended Data Fig. 2: CHARM shows minimal open-chromatin bias and enables single-cell analysis of bivalent chromatin.
From: Gene regulatory landscape dissected by single-cell four-omics sequencing
a, Pileup plot (top) and heatmap (bottom) showing chromatin accessibility signals at the TSS of expressed and unexpressed genes. b, Pileup plot (top) and heatmap (bottom) showing H3K27me3 signals at the TSS of expressed and unexpressed genes. c, Pileup plot (top) and heatmap (bottom) showing H3K27me3 signals at bivalent and silenced (neither expressed nor bivalent) promoters. d, Histogram of the odds ratio for co-occurrence of accessibility and H3K27me3 fragments within the same single cell at bivalent regions. e, Example of bivalent chromatin at single-allele resolution at the Gad2 locus. Each row represents an individual cell (n = 805), with the B6 and CAST alleles shown separately. A bin is labeled as “bivalent” when both accessibility and H3K27me3 signals are detected on the same allele within the same cell.
