Extended Data Fig. 4: Both cDC1 and cDC2 prime CD8 T cells after mRNA vaccination.
From: mRNA vaccines engage unconventional pathways in CD8+ T cell priming
a, Gating strategy for cDC1, cDC2, and B cells from LNs after mRNA-LNP immunization. b, Gating strategy for tetramer+ CD8 T cells from spleen. c, ELISpot results from cDNA vaccination experiment of Fig. 2d,e. P** = 0.0037, P*** = 0.0008, ns=0.8135. d, e, WT and Δ32 mice were injected i.m. with 10 μg OVA adjuvanted with AddaVax on day 0 and 7. Spleens were stained for SIINFEKL-Kb tetramer+ CD8 T cells and CD44/CD62L expression on day 11. Data represents pooled biologically independent samples from three independent experiments. (n = 15 WT. n = 9 Δ32). One-way ANOVA with Tukey’s multiple comparisons. P** = 0.0081. f, IFNg ELISpot results from Fig. 3e,f. (n = 7 Cre−, n = 8 Cre+). One-way ANOVA with Tukey’s multiple comparisons. g, SIINFEKL-Kb-PE MFI from tetramer+ CD8 T cells from the experiment shown in Fig. 2f,g. (n = 9 WT, n = 11 Δ32, and n = 11 Δ1+2+3). One-way ANOVA with Tukey’s multiple comparisons. h, SIINFEKL-Kb tetramer+ CD8 T cells on day 7 after mLama4 mRNA-LPX vaccination in WT, Δ32, and Δ1+2+3 mice. (unimmunized: n = 4; immunized: n = 8 WT, n = 7 Δ32, and n = 7 Δ1+2+3). One-way ANOVA with Tukey’s multiple comparisons. P*** = 0.0003. Data represented as mean values ± s.d. and pooled biologically independent samples from two independent experiments. ns = not significant.
