Extended Data Fig. 6: ROS-triggered oxidative stress disrupts hypoxia-responsive gene expression, without damaging the transcriptional machinery.
From: H2O2 repurposes plant O2 sensing to regulate post-hypoxia responses
(a) Relative expression of hypoxia-responsive and ROS-responsive genes in 35S:RAP2.3-nLuc seven-day-old seedlings in air or hypoxia (1% O2), for 6 h, followed by 3 h reoxygenation, upon 1 mM TBHP or mock treatment (n = 4). (b) Relative expression of hypoxia-responsive genes in seven-day-old Col-0 seedlings (n = 4) exposed to 6 h of air (mock), hypoxia (1%) alone, hypoxia combined with 1 mM TBHP, 10 mM ascorbate (Asc), or TBHP and Asc together (n = 4). (c) Relative expression of nLuc and hypoxia-responsive genes in seven-day-old HRPE:nLuc (35S-5’UTR) seedlings subjected to 6 h of air or hypoxia (1 % O2), followed by 3 h of reoxygenation upon TBHP or mock treatment (mock in air, hypoxia, reoxygenation and TBHP in air and hypoxia, n = 4; TBHP in reoxygenation, n = 3). Statistical analyses were conducted using one-way (b) or two-way (a, c) ANOVA followed by Tukey’s HSD test (p < 0.05). Different letters indicate statistically different groups. In a-c, boxplots indicate median (middle line), 25th and 75th percentiles (box limits), whiskers denote the 1.5x interquartile range; outliers are shown as individual points.
