Fig. 3: Oxidative stress increases ERFVII stability in a N-degron pathway-dependent manner.
From: H2O2 repurposes plant O2 sensing to regulate post-hypoxia responses
a, Relative nLuc activity of 35S:RAP2.3–nLuc seedlings treated under hypoxic (1% O2) or aerobic (21% O2) conditions for 6 h, followed by 3 h of reoxygenation, 1 mM TBHP or mock treatment (n = 4). b, Relative nLuc activity of 35S:RAP2.3–nLuc seedlings treated with different TBHP concentrations under aerobic conditions over 4 h (n = 6). c, Relative nLuc activity of 35S:RAP2.3–nLuc seedlings treated with 1 mM TBHP under aerobic conditions measured over 4 h (n = 6). d, Relative nLuc activity of the 35S:(C2A)RAP2.3–nLuc variant following 1 mM TBHP or mock treatment, under hypoxic (1% O2) or aerobic (21% O2) conditions (n = 4). e, Schematic representation of DLOR. f, Relative FLuc activity in S. cerevisiae cultures expressing AtPCO4 together with either C-DLOR, R-DLOR or D-DLOR, exposed to 0.5 mM TBHP or mock treatment for 30 min (n = 5). Statistical differences were evaluated using one-way (b and c) or two-way (a, d and f) ANOVA (P < 0.05) followed by Tukey’s HSD test (P < 0.05). In a–d and f, box plots indicate the median (middle line) and 25th and 75th percentiles (box limits); whiskers denote 1.5× the interquartile range, and outliers are shown as individual points.
