Extended Data Fig. 10: Usage of human CEACAM5, CEACAM6, and individual point mutants, by pseudotyped CcCoV-2A S.
From: Heart-nosed bat alphacoronaviruses use human CEACAM6 to enter cells
(a) Pseudovirus entry of CcCoV-2A S in HEK293T cells expressing the indicated CEACAM protein. The F63I substitution in CEACAM5 increases CcCoV-2A S-mediated entry to the level seen with CEACAM6. Values are presented as relative light units (RLU). Assays were performed twice in technical triplicates with mean RLU and SD shown. (b) CcCoV-2A S entry into cells expressing CEACAM6 substitution I63F is reduced relative to wild type human CEACAM6 (hCEACAM6). Assays were performed twice in technical triplicates in triplicate with percentage PV entry and SD shown. Statistical analysis was performed using paired one-way ANOVA (****p-value < 0.0001) and paired t-test (***p-value = 0.0002), respectively.
