Extended Data Fig. 5: Cas12a N-terminal amino acids are required for mRNA degradation by AcrVA2. | Nature

Extended Data Fig. 5: Cas12a N-terminal amino acids are required for mRNA degradation by AcrVA2.

From: Translation-dependent degradation of cas12 mRNA triggered by an anti-CRISPR

Extended Data Fig. 5: Cas12a N-terminal amino acids are required for mRNA degradation by AcrVA2.The alternative text for this image may have been generated using AI.

(a) mRNA levels were quantified for wildtype cas12a and mutant cas12a lacking the first 30 amino acids (but provided with a start codon) and normalized to rpoD. Expression is shown relative to acrIIA4 control. (b) RNA levels were quantified for wildtype and mutant cas12a and normalized to rpoD. Expression is shown relative to rfp control. For both (a) and (b), points represent technical triplicates (n = 3 technical replicates) from one representative qRT-PCR experiment calculated using the ΔΔCt method; mean ± standard deviation indicated. Each experiment was independently repeated at least twice with similar results.

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