Supplementary Figure 10: Deletion mutation limit of detection with locked nucleic acid probes.
From: Engineered immune cells as highly sensitive cancer diagnostics
Real-time qPCR amplification plots of CT26 and wildtype Balb/c genomic DNA show that the chromosome 7 (left) and 19 (right) deletions can be detected at allele frequencies of 0.1 and 1% respectively. Each condition is shown in triplicate. RFU, relative fluorescence units; AF, allele frequency.
