Supplementary Fig. 8: NRT1.1B and its natural variation modulate the assembly of the rice root microbiota.

(a,b) Principal coordinate analysis with unweighted (a) and weighted (b) UniFrac distance showing that the root microbiota of ZH11 (wild-type), nrt1.1b, Nipponbare NRT1.1B^indica, and Nipponbare NRT1.1B^japonica separate in the first two axes. Ellipses cover 68% of the data for each genotype. (c) Constrained principal coordinate analysis showing that 57.1% of the root microbiota variations are explained by genotypes (ZH11, nrt1.1b, NRT1.1B^indica, and NRT1.1B^japonica). The numbers of replicated samples are as follows: ZH11 (n = 16), nrt1.1b (n = 14), NRT1.1B^indica (n = 15), NRT1.1B^japonica (n = 15). (d,e) A full factorial replication experiment validates the conclusion that NRT1.1B and its natural variation modulate the assembly of the rice root microbiota. Unconstrained (d) and constrained (e) principal coordinate analysis with Bray-Curtis distance showing that the root microbiota of ZH11 (wild-type), nrt1.1b, NRT1.1B^indica, and NRT1.1B^japonica separate in the first two axes. The plants were grown in different fields and at different time from the samples in Fig. 4. Ellipses cover 68% of the data for each genotype. The numbers of replicated samples are as follows: ZH11 (n = 14), nrt1.1b (n = 10), NRT1.1B^indica (n = 15), NRT1.1B^japonica (n = 15).