Supplementary Fig. 11: The scheme for the previous high-throughput barcoding system to determine 16S rRNA gene sequences covering regions V5–V7 of bacterial isolates.
From: NRT1.1B is associated with root microbiota composition and nitrogen use in field-grown rice
A previously published two-step barcoded pyrosequencing procedure (Bai, Y. et al. Functional overlap of the Arabidopsis leaf and root microbiota. Nature 528, 364–369, 2015). Please note that the second step PCR generates chimera sequences that will contain mislabeled plate and well barcodes for bacterial identification.
