Supplementary Figure 3: Kinetics of antibody binding.
From: A coiled-coil masking domain for selective activation of therapeutic antibodies
Kinetics of binding for hBU12 and CC2B-hBU12 were assessed via biolayer interferometry using recombinant human CD19. Biotinylated antigen was immobilized on a streptavidin-coated biosensor and antibodies were then assessed for association and dissociation over time. Kinetic parameters were determined using the Octet analysis software using a 1:1 global fit binding model. This experiment was repeated in two independent experiments with similar results.
