Supplementary Figure 5: Proliferation of mouse T cells in the presence of either masked or unmasked anti-mouse CD3 antibodies.
From: A coiled-coil masking domain for selective activation of therapeutic antibodies
Mouse T-cells were labeled with carboxyfluorescein succinimidyl ester (CFSE) and incubated with either CC2B-masked or unmasked anti-mouse CD3 antibodies at 500 ng/mL along with anti-CD28 stimulation at 37 °C. T-cell activation was monitored after 4 days of incubation using flow cytometry. A leftward shift of fluorescence indicates that CFSE has been diluted as cells have proliferated. Robust proliferation was observed for the anti-mouse CD3 antibody whereas no proliferation occurred for the masked antibody. Two independent experiments were conducted with similar results.
