Supplementary Figure 6: Genome-wide specificity of EcoCascade-p300 targeted to IL1RN.
From: Targeted transcriptional modulation with type I CRISPR–Cas systems in human cells
(a) MA plot for ChIP-seq differential binding of Flag-tagged EcoCascade-p300 targeted to the IL1RN promoter with cr26 compared with binding of EcoCascade-p300 targeted to the IL1RN promoter with cr25 in HEK293T cells. Red data points indicate FDR < 0.001 by differential DESeq2 analysis using Wald test p-values. (b) MA plot for the differential expression of EcoCascade-p300 targeted by cr26 versus EcoCascade targeted by cr26 in HEK293T cells. Red data points indicate FDR < 0.01 by differential expression analysis using Wald test p-values. (n=3 biological independent samples).
