Supplementary Figure 2: Heterologous transfection of Cas constructs reveals optimized transactivation activity.
From: Targeted transcriptional modulation with type I CRISPR–Cas systems in human cells
(a) Schematic of the IL1RN locus along with IL1RN crRNA target sites. (b) Relative IL1RN expression following co-transfection of 100 ng individual crRNAs and 500 ng total EcoCascade with Cas6-p300 in various ratios. All samples processed at 3 days post transfection. (Tukey-test, *P<0.05, n=3 biological independent samples; error bars, SEM). Bars indicate mean relative expression. TSS, Transcription start site.
