Supplementary Figure 12: Proportional control of RhoA and Rac1 activation in HeLa cells.
From: Multi-input chemical control of protein dimerization for programming graded cellular responses
a,c, Change in normalized perimeter (a) or circularity (c) (DMSO baseline subtracted) over time (drug addition at 0 min) in HeLa cells expressing NS3a-CAAX with either DNCR2-TIAM and GNCR1-LARG, DNCR2-TIAM alone, or GNCR1-LARG alone. Lines are colored according to the drug conditions shown on the x-axis of the plot in (b). b,d, Change in normalized perimeter (b) or circularity (d) (average last 10 min-first 10 min). (a-d) Mean and s.e.m. of the number cells per condition listed in Supplementary Table 3 from four independent wells. e-g, Kernel density estimates of distributions of morphology statistics at 60 min post-drug addition in Hela cells expressing NS3a-CAAX with either (e) DNCR2-TIAM and GNCR1-LARG, (f) DNCR2-TIAM alone, or (g) GNCR1-LARG alone. h, Kernel density estimates of the distributions of morphology statistics at the first frame (before drug addition) for the three HeLa cell lines.
