Supplementary Figure 13: Yeast and human cell FACS gating strategies.
From: Multi-input chemical control of protein dimerization for programming graded cellular responses
a, Yeast gating for all events (left), expression positive events used to report median binding fluorescence values (middle), and example sorting gates (right). b, HEK293T gating for all events (left), followed by single cell gating (middle), and gating for transfection-positive cells (BFP positive, right). Untransfected cells (red), NS3aH1-BFP-dCas9-transfected cells (blue).
