Supplementary Figure 3: Grazoprevir:NS3a complex reader binding and improvement.
From: Multi-input chemical control of protein dimerization for programming graded cellular responses
a, Predicted mutational preferences of the G3 interface for binding to NS3a:grazoprevir, as defined by the frequencies of mutations found in Rosetta re-designs of the interface. b, Sequence logos of the theoretical library for the combinatorial library varying the G3 interface (top), and the mutations found in the final enriched library (bottom). Residue identities at the varied positions are indicated for the first-generation reader G3 and optimized reader GNCR1. c, Binding of 1 µM NS3a with avidity to yeast-displayed G3 or GNCR1 in the presence of grazoprevir, danoprevir, asunaprevir, or DMSO. Technical triplicates and their means are shown.
