Supplementary Figure 8: Single cell RNA-seq profiling of Pdia3 KO CD8+ T cells. | Nature Biotechnology

Supplementary Figure 8: Single cell RNA-seq profiling of Pdia3 KO CD8+ T cells.

From: In vivo CRISPR screening in CD8 T cells with AAV–Sleeping Beauty hybrid vectors identifies membrane targets for improving immunotherapy for glioblastoma

Supplementary Figure 8

(a) t-SNE plot of hierarchical clustering of scRNA-seq results. (b) Volcano plot of scRNA-seq of mouse CD8+ T cells after Pdia3 knockout. Pdia3 is a significantly downregulated gene after infection with AAV-sgPdia3. A total 9,193 single cells were captured and their transcriptomes were sequenced for the AAV-sgPdia3 (n = 4,549 single cells) and AAV-Vector (n = 4,644 single cells) treated CD8 T cells. Two-sided Wilcoxon signed-rank test by gene, with p-values adjusted by Benjamini & Hochberg.

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