Supplementary Figure 9: Immune marker analysis of PDIA3 KO human CD8⁺ T cells by CyTOF.

(a) t-SNE plot clustered by samples, showing that the 3 PDIA3 KO samples grouped with each other, the 3 WT samples also grouped with each other, and that PDIA3 KO samples and WT samples formed distinct groups. (b) t-SNE plot of CyTOF data with k-means clustering revealing 10 major clusters. (c) t-SNE and violin plots of CyTOF data of CD127 / IL7R, FAS / CD95, 4-1BB / CD137 and TIM-3 / HAVCR2 at the surface protein level, for both PDIA3 KO and wildtype single human CD8⁺ T cells (n = 3 replicates each, sampled 7,000 cells per replicate for comparison). Violins show kernel probability density on side, and boxplot is standard, i.e. middle band is median, hinges/ends of box are interquartile range (25% and 75% quantiles), lower whisker = smallest observation greater than or equal to lower hinge - 1.5 * IQR, upper whisker = largest observation less than or equal to upper hinge + 1.5 * IQR. Wilcoxon test, two-sided, p value adjusted by Benjamini & Hochberg method. KO vs WT: p = 1.91e-81 for CD127 / IL7R, p = 0.1147 for FAS / CD95, p = 6.75e-83 for 4-1BB / CD137, and p = 0 for TIM-3 / HAVCR2.