Supplementary Figure 2: Single cell RT-qPCR estimation of functional MOI of AAV-SB-CRISPR screen. | Nature Biotechnology

Supplementary Figure 2: Single cell RT-qPCR estimation of functional MOI of AAV-SB-CRISPR screen.

From: In vivo CRISPR screening in CD8 T cells with AAV–Sleeping Beauty hybrid vectors identifies membrane targets for improving immunotherapy for glioblastoma

Supplementary Figure 2

Single cell RT-qPCR detection of single T cell expressing functional sgRNAs, for the estimation of functional MOI with exact transduction parameters in the AAV-Surf screen. PBS treated single T cells were used as a negative control. Numbers of wells without T cells, with sgRNA- T cells and with sgRNA+ T cells were determined to estimate MOI.

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