Supplementary Figure 8: Sequence information of tRNA-gRNA recipient vector. | Nature Biotechnology

Supplementary Figure 8: Sequence information of tRNA-gRNA recipient vector.

From: Broad-spectrum resistance to bacterial blight in rice using genome editing

Supplementary Figure 8: Sequence information of tRNA-gRNA recipient vector.The alternative text for this image may have been generated using AI.

The intermediate vector pENTR4-U6.1P-ccdB/chl constructed as the recipient vector for tRNA-gRNA contains two Gateway recombination sequences (in blue), rice U6 promoter (in green), two BsaI (double underlined) sites. The cassettes of ccdB (in orange) and chl (chloramphenicol resistant) gene (in red) were constructed to facilitate the Golden Gate assembly of multiple tRNA-gRNA units.

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