Supplementary Figure 12: Molecular and phenotypic characterization of alleles of sweet13 mutants.

a, Number of seeds per panicle of greenhouse-grown wild-type sweet13-1 and -2 lines grown side by side (n = 4). No significant differences (P = 0.131 for sweet13-1 and P = 0.054 for sweet13-2) were observed with Student’s t-test. b, Total soluble sugars in wild-type and sweet13-1 and -2 flag leaves. Both mutants showed similar sugar concentrations compared to wild type. Samples were harvested at dusk (8 pm; n = 4 leaf samples from siblings grown in parallel and repeated independently at least three times, with similar results). Data were plotted using BoxPlotR (http://shiny.chemgrid.org/boxplotr/). Center lines show medians; box limits indicate the 25th and 75th percentiles as determined by R software; and data points were plotted as open circles. No significant differences were observed with Student’s t-test (sweet13-1: P = 0.318 for sucrose, P = 0.170 for glucose and P = 0.242 for fructose; sweet13-2: P = 0.824 for sucrose, P = 0.573 for glucose and P = 0.882 for fructose).