Supplementary Figure 13: mRNA levels of clade III SWEETs in sweet13 mutants.
a–c, Relative mRNA levels (2–ΔΔCt) of SWEET11, SWEET14 and SWEET15 in the sweet13-1 mutant (wild-type control: Kitaake). SWEET14 was the only SWEET clade III that showed significant upregulation in the mutant (mean ± s.e.m., n = 2 leaf samples from siblings grown in parallel, with mRNA levels normalized to the rice Ubiquitin1 levels, and repeated independently three times, with similar results). d, SWEET14 mRNA levels in the region around the laminar joint of the flag leaf (~1 cm of flag leaf blade base region plus laminar joint plus ~1 cm of flag leaf sheath top region) of the second allele sweet13-2 (in blue; wild-type control in white: Kitaake; center lines show the medians; box limits indicate the 25th and 75th percentiles as determined by R software; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles; outliers are represented by dots; and data points are plotted as open circles; n = 4 sample points).
