Supplementary Figure 12: Comparison of editing efficiency and DNA repair outcomes with FokI-PseCascade, FokI-dCas9, and wild-type Cas9.

Heat map (left) showing representative DNA repair outcomes at a set of target sites at the AAVS1 and CD34 loci with FokI–PseCascade, FokI-dCas9, and Cas9. FokI-PseCascade targets contained 30-32 bp interspacers and AAG PAMs, FokI-dCas9 targets contained 16 bp interspacers and NGG PAMs, and Cas9 was directed to the right half-site of each FokI-dCas9 target. Targets are shown on the x-axis, and sites with < 5% total indels were excluded from this analysis. Deletions lengths from 1–50-bp are in blue, and insertion lengths from 1–8 bp are in green; single bp insertions are separated by nucleotide identity, and the color intensity scales with frequency. The bar graph above the heat map displays total editing efficiency. Editing efficiency for all targets tested is shown at the top right, each dot represents the average of three nucleofection replicates at a single target site (except FokI-dCas9 target 4, which had two nucleofections), n=15 (FokI-dCas9, Cas9), 16 (FokI-PseCascade) unique target sites, bar graph and error bars report the mean and s.d. across sites.