Supplementary Figure 9: Evidence for gene editing in plants derived from the experiment described in Fig. 4b.

Plants were treated with constructs that included a single vector containing Wus2 and STM (Wus2/STM), a single vector containing Wus2 and ipt (Wus2/ipt), or a combination of co-inoculated vectors each containing a single DR (All combination, see Fig. 4 legend). Derived shoots were given individual designators to facilitate sample tracking. Purified genomic DNA was amplified for the PDS2 locus. Amplicons were pooled and submitted for next-generation sequencing. ‘Sequences analyzed’ denotes the observed number of sequences that contained the expected forward and reverse barcodes. ‘Insertions’ denotes the number of sequences observed to have non-specific DNA insertions at the sgRNA target site. ‘Deletions’ denotes the number of sequences observed to have targeted mutations at the sgRNA target site. ‘Indel frequency’ denotes the total number of sequences that were observed to have targeted modifications. ‘Observed mutations’ denotes mutations observed at a frequency >30% of the total. ‘Seed produced’ denotes those sampled shoots that produced seed. With the exception of plant 1-1-5, sequences of the resulting mutations are shown in Fig. 5b. Below the table are the spectra of mutations recovered for plant 1-1-5.