Supplementary Figure 1: Generating de novo meristems on seedlings.
From: Plant gene editing through de novo induction of meristems
a, Wus2- and STM-induced growths on 21–31% of treated seedlings. Few differences in frequency were observed among the different promoters used to express STM, probably because they all drive high expression in N. benthamiana. b–d, Approximately 12 d after initiating Fast-TrACC, callus-like growths formed, varying from one (b) to many (c,d) per seedling. e,f, Over the next 10–14 d, the growths expanded in size and remained in an undifferentiated state or began to differentiate. g,h, Shoot-like structures then formed, which could be excised and moved to rooting medium. The growths depicted are representative of those observed in three independent experiments.
