Supplementary Figure 8: Complementation of ETV2 knockout embryos (full term; n=3 biologically independent samples).

Body weights of all full term offspring were within range of normal birth weights (mean±SEM are 1.14±0.127 kg; n = 3). (a-k) Tissues were examined morphologically and then harvested for immunohistochemical analysis. a) The heart (open arrowhead) and descending aorta (arrowhead) were morphologically normal in the complemented animals. (b and c) The hearts were isolated and imaged to demonstrate the morphologically normal aortic arch in the complemented animals. (d and e) Wholemount images of the descending aorta demonstrate a longitudinal (d) section and cross sectional (e) image of the complemented aorta. Sections of skeletal muscle (f-j) and heart (j and k) were immunohistochemically stained for GFP (green) and the endothelial marker VWF (red). Note all the VWF endothelial cells were labeled with GFP. Scale bars for panels d-e = 2mm, panels f-g = 100μm, panels h-k = 50μm. (l) Quantification of chimeric rate by genomic PCR in indicated organs. ETV2 KO fibroblast and GFP Tg fibroblasts are parental cells used for cloning. Data shown are in triplicate for each tissue and plotted as mean±SEM..