Supplementary Figure 9: Evaluation of culture conditions for hiPSC-injected parthenotes.
From: Generation of human endothelium in pig embryos deficient in ETV2
(a) Development rate of parthenogenotes to morula (compaction) and blastocyst stages in three porcine media (n = 40 for each group). (b-g) Cell viability was assessed by TetraZ assay after culturing three hiPSC lines at indicated temperatures in NCSU-23 (b-d) or PZM-MU2 (e-g) media mixed with mTesRTM1 medium. Data shown are from n = 4 biologically independent experiments. (h) Number of porcine parthenogenotes containing live hiPSCs (shiPS9-1) over short-term culture (D4, n = 5; D5, n = 47; D6, n = 8 biologically independent samples). (i) Number of live hiPSC cells per parthenogenotes over short-term culture (D4, n = 5; D5, n = 47; D6, n = 8 biologically independent samples). Data represent mean±SEM. (j, k) Blastocyst rate of parthenogenotes injected with hiPSCs (SH91) at d4 or d6 after activation, scored at 24h (j) and 48h (k). Dot plots show results from n = 4-9 biologically independent experiments and plotted as mean±SEM.
