Supplementary Figure 13: BCL2 overexpression results in increased human:porcine chimerism.
From: Generation of human endothelium in pig embryos deficient in ETV2
(a) qPCR analysis was performed using genomic DNA from chimeric human:porcine embryos/embryo products at E17-E18 following delivery of hiPSCs (a) or BCL2-hiPSCs (b). Note the marked increase in efficiency using the BCL2-hiPSCs (compared to hiPSCs) in the top ten embryos. (c) and (d) Percentage of TIE2 positive cells among all the GFP positive cells and percentage of GFP positive cells among TIE2 positive cells, respectively in the human (BCL2-hiPSC):porcine chimeric embryos. (e) Comparison of Alu fold change using PCR in chimeric ETV2 null embryos that were complemented with either BCL2-hiPSCs (n = 63) or hiPSCs (n = 23) and transferred to surrogate gilts. The black dots represent individual samples in each group. The BCL2-hiPSC group emphasizes the increased efficiency of human-porcine chimerism compared to the hiPSCs group. The whisker plot shows all data points (minimum to maximum).
