Supplementary Figure 4: Targeting and genotyping strategies for generation of ETV2 knockout fibroblasts.
From: Generation of human endothelium in pig embryos deficient in ETV2
(a) Diagram of porcine (sus scrofa) ETV2 gene. Red arrows indicate location of guide RNAs and black arrows indicate genotyping primers. (b) Genotyping PCR. Deletion was confirmed by the absence of 5’ (5F1/5R1), 3’ (3F1/3R1), and internal (internal F1/R1) amplifications, and the presence of 5F1/3R1 amplification. 600 clones were screened and three independent clones with biallelic deletion of ETV2 were obtained and shown. (c) Expected fragment sizes for genotyping PCR are indicated.
