Supplementary Figure 5: Gating strategy used for FACS analyses.
From: Generation of human endothelium in pig embryos deficient in ETV2
For the analyses of flow cytometry experiments, the gating of acquired events was based on the forward scatter (FSC-A) and side scatter (SSC-A). Live cells were gated based on the propidium iodide (PI) negative and side scatter (SSC-A) plots. Singlets were gated based on the height and width of FSC (FSC-H vs. FSC-W) and SSC (SSC-H vs. SSC-W) for their cell size and granularity. Single cells stained with fluorescence-labeled antibodies specific for cell surface markers were analyzed and the data which were obtained were processed using FlowJo Ver10.4.2 software.
