Supplementary Figure 6: Porcine blastomeres integrate into parthenogenetic embryos and develop with the host.

(a) Schematic outline of the experimental scheme. Parthenogenetic embryos were generated by electrical stimulation of matured oocytes and cultured. At day 4, embryos reached the morula stage and were injected with 2–8 blastomeres expressing GFP. (b) Representative images of GFP-labeled blastomeres developing with the host parthenogenote. (c) Number of parthenogenotes containing GFP-labeled cells. The bar graph shows results obtained from three biologically independent (n = 3) experiments. (d) Number of GFP⁺ cells per parthenogenote. 80 parthenogenotes (n = 80) were injected with GFP⁺ blastomeres on D4. Nine out of 15 biologically independent parthenogenotes analyzed were positive for GFP expressing cells at D10. The p-value was determined by one-way ANOVA and non-parametric method with Brown-Forsythe test. Data represent mean±SEM (e) Immunohisochemistry revealed that GFP⁺ cells integrated and contributed to both OCT4⁺/CDX2⁺ (open arrowheads) and OCT4⁺/CDX2^- (filled arrowheads) populations. Data show representative images from n = 6 biologically independent samples.