Supplementary Fig. 2: Identification and validation of multiple cancer populations. | Nature Biotechnology

Supplementary Fig. 2: Identification and validation of multiple cancer populations.

From: Integrating microarray-based spatial transcriptomics and single-cell RNA-seq reveals tissue architecture in pancreatic ductal adenocarcinomas

Supplementary Fig. 2: Identification and validation of multiple cancer populations.The alternative text for this image may have been generated using AI.

(a) PDAC-A (top) and PDAC-B (bottom) CNV profiles inferred from scRNA-Seq (same as Fig. 1d, e). A subset of 200 randomly selected ductal cells were chosen as a negative control for the analysis and analyzed together with cancer clusters. (b) Expression levels of KRT19 (marker of malignant and non-malignant ductal epithelial cells), TM4SF1, and S100A4 projected onto t-SNE of PDAC-A (top) and PDAC-B (bottom). Note specificity of TM4SF1 expression for PDAC-A cancer cluster 1, and S100A4 expression for PDAC-A cancer cluster 2. In PDAC-B, TM4SF1 is expressed primarily by cancer cells whereas an S100A4 expressing cancer population is absent. (c) Double immunofluorescence staining (n = 2) for KRT19 and TM4SF1 in PDAC-A FFPE tissue. Note co-localization of KRT19 and TM4SF1 signal in malignant ducts (top panel, white arrowheads), but not in non-malignant ducts (bottom panel, white arrowheads). Scale bar, 250 µm. (d) Double immunofluorescence staining (n = 2) for KRT19 and S100A4 in PDAC-A FFPE tissue. Note co-localization of KRT19 and S100A4 signal in malignant ducts (top panel, white arrowheads), but not in non-malignant ducts (bottom panel, white arrowheads). Scale bar, 250 µm.

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