Supplementary Fig. 2: The allelic outcomes and indel value of STEMEs in rice protoplasts.
From: Targeted, random mutagenesis of plant genes with dual cytosine and adenine base editors
(a) The allelic outcomes of rice sites edited by STEME-1 (n=1 shown) in rice protoplasts. The simultaneous C>T and A>G editing efficiency of STEME-1 was ranged from 0.68-15.37% vs. 0.69-15.50% while only count the A>G substitutions (including single A>G substitution and simultaneous C>T and A>G substitutions) (Fig. 1d–f). (b) Indel frequencies of STEMEs and Cas9 in rice protoplasts. Comparison of indel frequencies by the A3A-PBE, PABE-7, STEME-1, STEME-2, STEME-3, and STEME-4 constructs in rice protoplasts. Values and error bars indicate the mean ± s.e.m of three independent experiments.
