Extended Data Fig. 10: Characterization of hiPS cells derived from subjects with 22q13.3 deletion syndrome. | Nature Biotechnology

Extended Data Fig. 10: Characterization of hiPS cells derived from subjects with 22q13.3 deletion syndrome.

From: Generation of human striatal organoids and cortico-striatal assembloids from human pluripotent stem cells

Extended Data Fig. 10: Characterization of hiPS cells derived from subjects with 22q13.3 deletion syndrome.The alternative text for this image may have been generated using AI.

(a) Representative images showing morphology of hiPS cells from control and 22q13.3DS patients. Scale bars: 1 mm. Imaging was repeated in 2 independent differentiation experiments with similar results. (b) SNP array of hiPS cells showing the 22q13.3 deletion locus. Upper shows B Allele Frequency, and bottom Log R Ratio for chromosome 22q region. (c) Immunostaining for pluripotency stem cell markers OCT4 (green) and SSEA4 (magenta). Scale bars: 200 μm. (d) Representative images of 3D neural spheroids at day 5 of differentiation from control and 22q13.3DS hiPS cell lines. Scale bars: 1 mm. Imaging was repeated in spheroids from 3 independent differentiation experiments with similar results. (e) Gene expression (RT-qPCR) for the forebrain marker FOXG1, the LGE markers GSX2, MEIS2, BCL11B (CTIP2), and the spinal cord marker HOXB4 at day 22 of differentiation in hCS and hStrS derived from 22q13.3DS hiPS cells (n = 9 neural spheroids from 3 hiPS cell lines; two-tailed Mann-Whitney test **P < 0.002 for FOXG1, two-tailed unpaired t-test ***P = 0.0001 for GSX2, two-tailed Mann-Whitney test ****P < 0.0001 for MEIS2, two-tailed unpaired t-test ****P < 0.0001 for BCL11B (CTIP2), two-tailed Mann-Whitney test P = 0.99 for HOXB4. (f) Immunostaining for DARPP32 (green), CTIP2 (magenta) and NeuN (blue) in day 85 hStrS. Scale bar: 50 μm. Data shown are mean ± s.e.m. Immunostainings were repeated in spheroids from 2 independent differentiation experiments with similar results.

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