Extended Data Fig. 8: Long-term effects of recombinant Lon protease treatment in the MB49 bladder cancer model.

a-d, Prolonged protection was detected in recombinant Lon protease (rLon) treated mice at follow-up without further treatment (27 days) and with further treatment of rLon twice per week (35 days) compared to Sham, which were sacrificed on day 12 (n = 15 Sham and rLon follow-up, n = 12 rLon long-term, n = 2 experiments). a, A decrease in pathology score was detected in rLon treated mice after 27 days and after 35 days. b-d, Lower bladder weight in rLon treated mice at follow-up (27 days) and after continued treatment (35 days) compared to Sham (day 12). Smaller bladder size in mice receiving continued treatment compared to Sham (day 12). e, Heat-map showing a lower number of regulated genes in the follow-up and long-term groups compared to Sham (red = up-regulated, blue = down-regulated, black = not significantly regulated, cut off FC ≥ 2, P < 0.05, n = 2 per group). Data are presented as mean ± s.d. and analysed by two-tailed Kruskal-Wallis test with Dunn’s correction. f, Top regulated genes associated with the Molecular Mechanisms of Cancer pathways were strongly inhibited by rLon treatment at follow-up and further in the long-term treatment group.