Supplementary Figure 8: TapeStation quantification of DNA fragments obtained from healthy adult samples.
From: Complete, closed bacterial genomes from microbiomes using nanopore sequencing
(a) TapeStation quantification of DNA extracted from healthy adult stool samples A (green), C (blue), and E (yellow), prior to size selection. (b) TapeStation quantification of samples from panel A, after size selection with SPRI beads. All but one sample (A, green) yielded very short fragments and insufficient DNA after size selection. (c) TapeStation quantification of DNA extracted from eight healthy adult stool samples (A, B, C, E, F, G, H, J) after extraction with the present approach shows a significant enrichment for DNA fragments above 10 kb and minimal shorter fragments. Extractions were performed once per sample.
