Fig. 2: Per-organism assembly contiguity, diversity and taxonomic read composition in two healthy human stool microbiomes.
From: Complete, closed bacterial genomes from microbiomes using nanopore sequencing
a, Species-level Shannon diversity is shown for the sequence datasets obtained. Higher diversity is found in libraries prepared with the present DNA extraction method. Relative species-level abundances are shown for a conventional workflow consisting of bead-beating extraction and short-read sequencing, as well as the present workflow consisting of HMW DNA extraction and long-read sequencing. b, Contiguity is expressed as per-bin N50 divided by per-bin length (the total length of sequences assigned to the bin). As bin assembly approaches completion, the quantity N50 divided by bin length approaches one, regardless of genome size. Nanopore sequencing and assembly (blue, purple) demonstrates higher assembly contiguity than read-cloud (gold) and short-read (green) approaches. For all organisms achieving assembly N50 of at least 500 kbp or a complete genome draft by any approach, genome draft quality and contiguity are shown for long reads, read clouds and short reads. Shapes indicate draft quality. Asterisk marks a genome later annotated as putative Cibiobacter.
