Supplementary Figure 10: Reproducibility of the DSP system.
From: Multiplex digital spatial profiling of proteins and RNA in fixed tissue
a, Serial sections of tonsil tissues analyzed in Fig. 3a. ROIs were selected across each of the tissues (n=36), and the overlapping ROIs (n=25) were used for this analysis. Sections were stained with CD3, CD20, and PanCK antibodies directly conjugated to fluorescent dyes. b, Correlation of CD3, CD20, and PanCK on both serial sections across the 25 overlapping ROIs. c, Four ROI types selected, based on visualization marker expression, for correlation analysis of the 44 antibodies in the cocktail. d, Correlation of antibody counts across overlapping ROIs (n=25) on adjacent sections.
