Supplementary Figure 3: Induction efficiency of CD177+ and CD275+ ADE shows variation using different protocols. | Nature Biotechnology

Supplementary Figure 3: Induction efficiency of CD177+ and CD275+ ADE shows variation using different protocols.

From: Generation of pancreatic β cells from CD177+ anterior definitive endoderm

Supplementary Figure 3

(a-c) Adaptation of previously published endoderm differentiation protocols from hESCs. (d) FACS quantification for the percentage of total population expressing CXCR4 in DE cells derived from HMGUi001-A hiPSC using 3 different endoderm induction protocols (n = 2 biologically independent experiments). (e) FACS quantification for the percentage of cells expressing CXCR4+/CD177+ and CXCR4+/CD275+ in DE generated using previously published protocols (n = 3 (HMGUi001-A hiPSC), n = 2 (H9) biologically independent experiments). Data is represented as mean ±s.e.m.

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