Extended Data Fig. 5: CaTCH reveals a strong clonal selection of RAFi/MEKi-treated melanoma cells in vivo.
From: Isolating live cell clones from barcoded populations using CRISPRa-inducible reporters
a, Respective survival curves of resistance generation experiment in vivo from Fig. 2b. Statistical analysis was performed by two-sided Log-rank (Mantel-Cox) test, comparing RAFi-MEKi-treated to untreated. **P < 0.01, ns = non-significant. (Untreated = 4 tumors isolated from 4 mice; RAFi/MEKi = 5 tumors isolated from 5 mice). b, Violin plot showing the distribution of identified BCs within samples. REF = starting cell line, T1-T9 = individual tumors, one tumor per mouse; median:line, middle 50% of data: box, 1.5*IQR: whiskers; individually depicted points indicate outliers; the exact number of barcodes per individual tumor can be derived from Fig. 2c. c, Pearson correlation matrix of all identified BCs, n = 58269 unique BCs. d, Heatmap showing normalized read-counts of selected BCs in the indicated samples.
