Fig. 1: HFOs recapitulate patterns of early cardiomyogenesis.
From: Human heart-forming organoids recapitulate early heart and foregut development
a, The protocol for HFO formation. hPSC aggregates were individually embedded in Matrigel and differentiated using CHIR and IWP2. The figure shows the development of HFOs from d−4 until d10 of differentiation (upper row) as well as MIXL1–GFP and NKX2.5–eGFP expression patterns (bottom row). b, A typical HES3 NKX2.5–eGFP-derived HFO forming three layers: IC, ML and OL. c, An HFO rotated around its virtual axis and respective schemes. d, Whole-mount IF staining for the myocardial marker MHC. e, A paraffin section stained for cTnT and an anti-GFP antibody. f, A scheme of an HFO with highlighted cardiomyocytes. g, Sequential paraffin sections stained for cTnT and the ST marker WT1. h, A scheme of an HFO with highlighted ST-like cells. i, Whole-mount IF staining for the mesenchymal cell marker vimentin. j, A scheme of an HFO with highlighted mesenchymal cells. Scale bars, 500 µm (a) and 200 µm (b–e,g,i).
