Extended Data Fig. 7: Defining the myocardial layer compactness of control versus NKX2.5-KO HFOs.
From: Human heart-forming organoids recapitulate early heart and foregut development

The myocardial layer (ML) compactness was defined as the eGFP-positive area in proportion to the ML area. The figure shows stepwise how the compactness was determined using ImageJ software. Scale bars: 500 µm.