Fig. 3: Differential expression of SOX17, SOX2 and HNF4α reveals formation of distinct foregut endoderm tissues. | Nature Biotechnology

Fig. 3: Differential expression of SOX17, SOX2 and HNF4α reveals formation of distinct foregut endoderm tissues.

From: Human heart-forming organoids recapitulate early heart and foregut development

Fig. 3: Differential expression of SOX17, SOX2 and HNF4α reveals formation of distinct foregut endoderm tissues.

a, H&E staining (left column) and staining for the endoderm marker SOX17 (right column) with enlargements showing endodermal cavities (END CAV) in the IC and endodermal islands (END ISL) in the OL. b, TEM images of endodermal cavities in the IC with microvilli (arrows). c,d, Cryosections of HFOs stained for cTnT and the AFE marker SOX2 (c) or the hepatocyte marker HNF4α (d). The arrows point at endodermal islands in the OL. e, A scheme of an HFO with highlighted distinct endoderm tissues. Scale bars, 100 µm (a), 5 µm (b) and 200 µm (c,d).

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