Fig. 5: NKX2.5-KO HFOs recapitulate aspects of the respective phenotype in mice.
From: Human heart-forming organoids recapitulate early heart and foregut development
a, NKX2.5-KO organoids appear less compact than control HFOs as shown on whole HFOs (first row) and on paraffin sections stained for cTnT (second and third rows). b–d, A comparison of ML compactness (b), total area (c) and cardiomyocyte (eGFP-positive cell) area (d) between control and NKX2.5-KO organoids on d10 of differentiation applying a two-tailed t-test. b, Control: n = 14 HFOs, NKX2.5 KO: n = 14 HFOs from 3 independent experiments; c, control: n = 10 HFOs, NKX2.5 KO: n = 10 HFOs from 2 independent experiments; d, control: n = 109 cells from 3 HFOs, NKX2.5 KO: n = 133 cells from 4 HFOs derived from 1 experiment; the data are presented as mean ± s.e.m.; ****P ≤ 0.0001. e, TEM images of sarcomeres (arrows) in control and NKX2.5-KO organoids. Scale bars, 100 µm (a) and 1 µm (e).
