Fig. 3: Adapting the assay for whole blood and saliva sample types.
From: Engineering luminescent biosensors for point-of-care SARS-CoV-2 antibody detection
a, spLUC assays can be accomplished in as few as 5 min. CR3022 (10 nM) was incubated with S sensors for 5, 10, 15 or 20 min. Luciferase substrates were then added and incubated with the reaction mix for 0, 2, 4, 6, 8 or 10 min. All reactions showed bright luminescence signal. Each dot represents the average of two technical duplicates from n = 1 independent experiment. Lines connecting the means of the samples are plotted. b, The spLUC assay is compatible with whole blood samples and shows similar signal in the corresponding plasma samples with both fresh and lyophilized sensors. Each dot represents the average of two technical replicates from n = 1 independent experiment. A non-parametric Spearman correlation analysis was performed, and R = 0.94 was observed for S sensors, and R = 1 and 0.98 were observed for N sensor fresh and lyophilized sensors, respectively. c, Anti-S antibodies were detected in saliva samples with moderate sensitivity (33/42, 79%). The signals from saliva samples positively correlated with corresponding serum samples. Each dot represents the average of two technical replicates from n = 1 independent experiment. A non-parametric Spearman correlation analysis was performed, and the R value (0.66) and P value (<0.0001, two-tailed) are labeled in the graphs. The line represents linear regression.
