Extended Data Fig. 9: REDMAP-mediated SEAP production in animals.
From: A small and highly sensitive red/far-red optogenetic switch for applications in mammals
a, HEK-293 cells transfected with REDMAP system were microencapsulated into coherent alginate-poly- (L-lysine)-alginate beads (400 μm; 200 cells per capsule) and subcutaneously implanted into mice, rats, and rabbits. The animals were illuminated from the top with red light (20 mW/cm2) and blood SEAP was quantified 48 h after the first illumination. b–d, Light-induced SEAP expression in mice (b), rats (c), and rabbits (d). b, Mice implanted with 2.5×106 microencapsulated transgenetic HEK-293 cells and illuminated daily for 1 min. ***P < 0.0001. c, Rats implanted with 1.2×107 microencapsulated transgenetic HEK-293 cells and illuminated twice daily for 5 min. ***P < 0.0001. d, Rabbits implanted with 3×107 microencapsulated HEK-293 transgenic cells and illuminated twice daily for 5 min. Control animals were implanted with microencapsulated wild-type HEK293 cells. ***P < 0.0001. Data are presented as means ± SEM (n = 5 animals). P values were calculated by two-tailed unpaired t test. N.S., not significant.
