Extended Data Fig. 1: Characterization of the performance of the REDMAP system.
From: A small and highly sensitive red/far-red optogenetic switch for applications in mammals
a, SEAP production of HEK293 cells transfected with REDMAP-encoding plasmids. HEK293 cells transfected with REDMAP-encoding plasmids were illuminated with red light (660 nm, 1 mW/cm2) for 1 min. The control cells were transfected with different expression vectors to yield different combinatorial configurations for the presence (+) or absence (-) of ΔPhyA-Gal4, FHY1-VP64 and SEAP reporter. ***P < 0.0001. b, Constitutive gene expression of human cells exposed to far-red, red and blue light, respectively. HEK293 cells were transfected with 100 ng pSEAP2-Control, followed by illumination (1 mW/cm2, 660 nm, 730 nm, or 465 nm) for 1 h. ***P < 0.0001. c, Viability of human cells exposed to far-red, red and blue light, respectively. HEK293 cells bearing the REDMAP system were illuminated with different wavelengths of light (1 mW/cm2, 660 nm, 730 nm, or 465 nm) for 1 h. ***P < 0.0001. d, REDMAP–mediated transgene expression in different mammalian cell lines. Different mammalian cell lines were co-transfected with REDMAP-encoding plasmids. Twenty-four hours after transfection, cells were illuminated with red light (1 mW/cm2) for 1 min, and SEAP expression in the culture supernatant was scored 24 h after illumination. Data are expressed as means ± SD; n = 3 independent experiments. P values were calculated by two-tailed unpaired t test. N.S., not significant. Detailed description of genetic components and transfection mixtures are provided in Supplementary Table 1 and 5.
