Extended Data Fig. 5: Evolved DddA variants mediate mitochondrial base editing in multiple human cell lines. | Nature Biotechnology

Extended Data Fig. 5: Evolved DddA variants mediate mitochondrial base editing in multiple human cell lines.

From: CRISPR-free base editors with enhanced activity and expanded targeting scope in mitochondrial and nuclear DNA

Extended Data Fig. 5: Evolved DddA variants mediate mitochondrial base editing in multiple human cell lines.

a-c, Mitochondrial DNA editing efficiencies of canonical and evolved ND5.2-DdCBE in (a) HeLa cells, (b) K562 cells, and (c) U2OS cells. Editing efficiencies were measured for unsorted cells (bulk) and isolated DdCBE-expressing cells (sorted). Target spacing region and split DddA orientation are shown. Cytosines highlighted in light purple and dark purple are in non-TC contexts. Values and error bars reflect the mean±s.d of n = 3 independent biological replicates.

Back to article page