Extended Data Fig. 6: Mitochondrial genome-wide off-target C•G-to-T•A mutations. | Nature Biotechnology

Extended Data Fig. 6: Mitochondrial genome-wide off-target C•G-to-T•A mutations.

From: CRISPR-free base editors with enhanced activity and expanded targeting scope in mitochondrial and nuclear DNA

Extended Data Fig. 6: Mitochondrial genome-wide off-target C•G-to-T•A mutations.

Average frequency and mitochondrial genome position of each unique C•G-to-T•A single nucleotide variant (SNV) is shown for HEK293T cells treated with (a) canonical ATP8-DdCBE, (b) ATP8-DdCBE containing DddA6, (c) ATP8-DdCBE containing DddA11, (d) canonical ND5.2-DdCBE, (e) ND5.2-DdCBE containing DddA6 and (f) ND5.2-DdCBE containing DddA11. g, Ratio of average on-target:off-target editing for the indicated canonical and evolved DdCBE. The ratio was calculated for each treatment condition as: (average frequency of all on-target C•G base pairs)÷(average frequency of non-target C•G base pairs present in the mitochondrial genome).

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