Extended Data Fig. 10: Assessment of the specificity and sensitivity of the anti-idiotype for 3BNC117.
From: In vivo engineered B cells secrete high titers of broadly neutralizing anti-HIV antibodies in mice
a. Experimental scheme. Cells are engineered a day following extraction from spleen and activation. We used three different donor AAVs, each expressing a different antibody: either 3BNC117, VRC01 or 10−1074. b. Flow cytometry of gp120 or anti-idiotype binding of engineered cells, two days following treatment. Staining procedure is indicated above the plots. FcR block was used in staining. Each row indicates a different AAV used. Untransduced cells serve as the negative control. c. Quantification of B. ****; pv < 0.0001 for Two-Way ANOVA with Tukey’s multiple comparison.
